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SRX4775078: GSM3406076: Neg_control_1_2; Mus musculus; OTHER
1 ILLUMINA (Illumina HiSeq 4000) run: 87.2M spots, 8.9G bases, 3.1Gb downloads

Submitted by: NCBI (GEO)
Study: IKZF2 is required for myeloid leukemic stem cells by driving self-renewal and inhibiting the myeloid differentiation program II
PRJNA493794 • SRP162854 • All experiments • All runs
show Abstracthide Abstract
We report that IKZF2 is required for maintaining chromatin accessibility in leukemic stem cells in myeloid leukemia. RNA seq and ATAC-seq were performed to investigate the changes in chromatin accessibility of differentially expressed genes in leukemic stem cells when IKZF2 was absent. We found that IKZF2 maintains open accessibility in self-renewal transcription factor motifs such as HOXA9 sites whereas motifs of differentiation transcription factors including C/EBPs are kept closed. Overall design: 3 Ikzf2 CUT&RUN, each 2 technical replicates; 2 Negative control, each 2 technical replicates
Sample: Neg_control_1_2
SAMN10142592 • SRS3855678 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 4000
Strategy: OTHER
Source: GENOMIC
Selection: other
Layout: PAIRED
Construction protocol: DNA was extracted using spin column. barcoded library for Illumina sequencing was generated using with Tru-Seq adapters. 60 million reads using paired end Illumina sequencing was performed.
Experiment attributes:
GEO Accession: GSM3406076
Links:
Runs: 1 run, 87.2M spots, 8.9G bases, 3.1Gb
Run# of Spots# of BasesSizePublished
SRR793997787,219,5638.9G3.1Gb2018-10-01

ID:
6459283

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